Transport of 5-methyltetrahydrofolic acid and folic acid in freshly isolated hepatocytes.
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منابع مشابه
Transport of 5-methyltetrahydrofolic acid and folic acid in freshly isolated hepatocytes.
Transport of 5-methyltetrahydropteroylglutamic acid (5CH,-H,PteGlu) was studied in hepatocytes isolated from rat liver by enzymatic dispersion. The hepatocytes maintained gradients for sodium and potassium ions (I Na+l = 28.1 meql liter and I K+I = 133 meqlliter of cell water) during 90 min of incubation. Uptake of 5-CH,,-H,PteGlu was found to be a complex process. At low concentrations of subs...
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A sensitive method based on high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the simultaneous determination of folic acid (FA) and its active metabolite, 5-methyltetrahydrofolic acid (5-M-THF), in human plasma. The analytes were extracted from plasma with methanol solution containing 10 mg/mL of 2-mercaptoethanol and 0.025% (v/v) ammonium hydroxi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34833-0